Repligen MiniKros Hollow Fiber Filter 750 kD 1600 cm2 41.5 cm

Description

Repligen MiniKros S04-E750-05-N Hollow Fiber Filter (750 kDa, 1600 cm²)

The Repligen S04-E750-05-N is a high-efficiency MiniKros Sampler hollow fiber module designed for pilot-scale Tangential Flow Filtration (TFF). Utilizing proprietary modified Polyethersulfone (mPES) membrane technology, this filter offers exceptional flux rates and high product recovery with minimal non-specific binding. With a 750 kDa MWCO and a significant 1600 cm² surface area, it is the industry standard for the concentration and diafiltration of large biopharmaceuticals, including vaccines, viral vectors, and large multi-meric proteins. The 41.5 cm effective length and 0.5 mm fiber ID are engineered to provide optimal shear conditions, ensuring gentle processing of shear-sensitive biological products while maximizing throughput.

Technical Performance and Design

• Advanced mPES Membrane: The modified Polyethersulfone chemistry provides a hydrophilic, low-fouling surface that outperforms standard PES in both water permeability and product yield (>95% recovery typical).

• Optimized Hollow Fiber Geometry: The 0.5 mm lumen (Fiber ID) creates a precise flow path that reduces concentration polarization, allowing for faster processing of high-viscosity or high-concentration feeds.

• 750 kDa Selective Barrier: Specifically tuned for “Large-Molecule” Ultrafiltration. It effectively retains viral particles (AAV/Lenti) and large proteins while allowing smaller impurities and buffer components to pass into the permeate.

• Scalable Performance: Part of the Spectrum® filtration line, the S04 series allows for seamless scale-up from R&D to cGMP production by maintaining consistent flow-path lengths and membrane chemistry.

Product Specifications

• Catalog Number: S04-E750-05-N

• Module Family: MiniKros Sampler

• MWCO: 750 kDa

• Surface Area: 1600 cm²

• Effective Length: 41.5 cm (Total length 47 cm)

• Fiber ID: 0.5 mm (250 Fibers)

• Inlet/Retentate Connections: 3/4″ Sanitary Flange (TC)

• Permeate Connections: 3/4″ Sanitary Flange (TC)

• Condition: Dry (Non-sterile version; also available as -S for Sterile)

Key Benefits & Applications

• Viral Vector Purification: Highly validated for the concentration of AAV and Lentiviral vectors, where the 750 kDa pore size provides the perfect balance of impurity removal and high viral titer recovery.

• Exosome & Nanoparticle Processing: Ideal for the clarification and washing of extracellular vesicles (EVs), liposomes, and other synthetic nanoparticles.

• Continuous Bioprocessing: The high surface area allows for extended run times in perfusion or continuous harvest applications without significant flux decay.

• Ease of Integration: Standard 3/4″ TC fittings allow for quick “plug-and-play” connection to KrosFlo® systems or other TFF skids.

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