ACEA Analyzer and E-Plate Station

Description

ACEA Biosciences RT-CES xCELLigence System – W200 Analyzer & 16× RT-CIM E-Plate Station (MPN: 100016-001)

The ACEA Biosciences 100016-001 (now under the Agilent Technologies portfolio) is a high-performance, label-free microelectronic cell sensor system designed for real-time cellular analysis (RTCA). The system pairs the compact W200 RT-CES (Real-Time Cell Electronic Sensing) Analyzer with a specialized 16× RT-CIM E-Plate Cradle Station. Utilizing proprietary microelectrode impedance technology, this system enables non-invasive, continuous monitoring of cell proliferation, morphology changes, attachment strength, and cell invasion or migration processes without the need for destructive fluorescent or radioactive labeling reagents.

Technical Performance and Design

• Impedance-Based Cell Index Tracking: Features integrated gold microelectrodes patterned onto the bottom of specialized E-Plates. As cells attach and spread across the electrode array, they alter the electrical impedance, generating a dynamic, multi-frequency “Cell Index” value in real time.

• Dual Functional Assay Horizons: The system interfaces natively with both standard 16-well E-Plates (for routine cell proliferation, cytotoxicity, and cell-adhesion assays) and dual-chamber CIM-Plate 16 modules (for automated cell migration and invasion profiling).

• Incubator-Safe Station Footprint: The 16× RT-CIM station is engineered with specialized moisture-resistant alloy casings, allowing it to be placed directly inside standard CO_2 incubators under high-humidity conditions (98% RH} while routing data externally to the analyzer module via flat ribbon lines.

• Label-Free, Non-Invasive Diagnostics: Eliminates endpoint-only assays and toxic chemical dyes. Because the low-voltage output test signal ($22text{ mV RMS}$) is completely harmless to living tissue, cells remain biologically intact for downstream endpoint validations, Western blots, or genomic extractions.

Product Specifications

• Catalog Number / MPN: 100016-001

• Manufacturer / Brand: ACEA Biosciences / Agilent Technologies (xCELLigence® Line)

• System Configuration: W200 RT-CES Core Analyzer + 16-Well RT-CIM E-Plate Station

• Plate Capacity: One 16-well E-Plate, E-Plate VIEW 16, or CIM-Plate 16 per run

• Measurement Frequency Range: Multi-frequency switching (10 kHz, 25 kHz, and 50 kHz capabilities)

• Impedance Repeatability Metric: Accurate to within ±1.5% under stable climate profiles

• Communication Interface: RS-232 / USB Virtual Serial mapping at 57,600 bits/second

• Power Requirements: Universal 100–240 VAC, 50/60 Hz autosensing input

Key Benefits & Applications

• Cancer Cell Invasion & Migration: The gold standard framework for tracking the kinetic movement of metastatic cancer lines through simulated extracellular matrices in Transwell-style assays.

• In Vitro Cytotoxicity & Drug Screening: Perfect for charting the exact time-dependent toxicity profile of candidate small molecules or immunotherapies without missing early-onset apoplectic events.

• Receptor-Mediated Cell Signaling: Captures rapid, transient morphological adjustments triggered by G-protein coupled receptors (GPCRs) or growth factor signaling pathways within seconds of ligand addition.

• Viral Cytopathic Effect (CPE) Kinetics: Utilized by virology research cores to continuously plot viral infection kinetics and neutralizer antibody efficacy curves without requiring plaque staining assays.

Used. Excellent condition. 

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