Description
Description
Ethidium Bromide ~95% HPLC 10 Gram
Synonym: 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide, EtBr, Homidium bromide
- CAS Number 1239-45-8
- Empirical Formula (Hill Notation) C21H20BrN3
- Molecular Weight 394.31
- Beilstein/REAXYS Number 3642536
- EC Number 214-984-6
- MDL number MFCD00011724
- PubChem Substance ID 24894683
- NACRES NB.22
| Quality Level | 200 |
| assay | ~95% (HPLC) |
| mp | 260-262 °C (dec.) (lit.) |
| SMILES string | [Br-].CC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14 |
| InChI | 1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H |
| InChI key | ZMMJGEGLRURXTF-UHFFFAOYSA-N |
Application
Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.
Frameshift mutagen which intercalates double-stranded DNA and RNA.
GHS06, GHS08
Signal word Danger
Hazard statements H302-H330-H341
Precautionary statements P201-P260-P264-P280-P304 + P340 + P310-P403 + P233
Personal Protective Equipment Eyeshields, Faceshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges
RIDADR UN 2811PIH 6.1 / PGI
WGK Germany 3
RTECS SF7950000
Documents
Safety Data Sheet
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