Description
Description
Dl-Dithiothreitol For Electrophoresis ≥99% 5 Gram
Synonym:threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT
- CAS Number 3483-12-3
- Linear Formula HSCH2CH(OH)CH(OH)CH2SH
- Molecular Weight 154.25
- Beilstein/REAXYS Number 1719757
- EC Number 222-468-7
- MDL number MFCD00004877
- PubChem Substance ID 24894266
- NACRES NB.22
| grade | for electrophoresis |
| assay | ≥99% |
| mp | 40-44 °C |
| 41-44 °C (lit.) | |
| solubility | H2O: 50 mg/mL, clear, colorless to very faintly yellow |
| cation traces | heavy metals (as Pb): ≤10 ppm |
| absorption | ≤0.04 at 280 at 0.02 M |
| storage temp. | 2-8°C |
| SMILES string | O[C@H](CS)[C@H](O)CS |
| InChI | 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1 |
| InChI key | VHJLVAABSRFDPM-QWWZWVQMSA-N |
Application
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
Analysis Note
Tested for use in reduced SDS-PAGE
GHS07
Signal word Warning
Hazard statements H302-H412
Precautionary statements P264-P270-P273-P301 + P312 + P330-P501
Personal Protective Equipment dust mask type N95 (US), Eyeshields, Faceshields, Gloves
RIDADR UN 3335
WGK Germany 3
RTECS EK1610000
Flash Point(F) 235.4 °F
Flash Point(C) 113 °C
Documents
Safety Data Sheet
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