Repligen MiniKros Hollow Fiber Filter Sterile 750 kDa 1600 cm2

Description

Repligen MiniKros S04-E750-05-S Hollow Fiber Filter (Sterile, 750 kDa, 1600 cm2)

The Repligen S04-E750-05-S is a high-performance MiniKros Sampler hollow fiber module designed for pilot-scale Tangential Flow Filtration (TFF) under aseptic conditions. Utilizing proprietary modified Polyethersulfone (mPES) membrane technology, this filter offers exceptional flux rates and high product recovery with minimal non-specific binding. This “S” designation indicates the module is supplied Gamma Irradiated (Sterile), providing a closed-system solution for sensitive bioprocessing workflows. With a 750 kDa MWCO and a significant 1600 cm2 surface area, it is the industry standard for the concentration and diafiltration of large biomolecules, including vaccines, viral vectors, and nanoparticles.

Technical Performance and Design

• Aseptic Ready (-S): Gamma irradiated for immediate integration into sterile flow paths, eliminating the need for user-led sanitization cycles prior to processing sensitive biological samples.

• Advanced mPES Membrane: The modified Polyethersulfone chemistry provides a hydrophilic, low-fouling surface that maximizes water permeability and ensures high product yield (>95% typical recovery).

• Optimized Hollow Fiber Geometry: The 0.5 mm fiber ID (lumen) provides a precise flow path that balances shear forces with throughput, making it ideal for shear-sensitive viruses and large proteins.

• 750 kDa Selective Barrier: Engineered for “Large-Molecule” Ultrafiltration. It effectively retains viral particles (AAV/Lenti) while allowing smaller host-cell proteins and buffer components to pass into the permeate.

Product Specifications

• Catalog Number: S04-E750-05-S

• Module Family: MiniKros Sampler

• MWCO: 750 kDa

• Surface Area: 1600 cm2

• Effective Length: 41.5 cm

• Fiber ID: 0.5 mm

• Sterility: Gamma Irradiated (Sterile)

• Connections: 3/4″ Sanitary Flange (TC) for Inlet, Retentate, and Permeate ports.

Key Benefits & Applications

• Viral Vector Concentration: Highly validated for the concentration of AAV and Lentiviral vectors in gene therapy research, where sterile integrity and high viral titer recovery are critical.

• Vaccine Manufacturing: Ideal for the purification of large viral antigens and recombinant protein vaccines at the pilot scale.

• Exosome & Liposome Processing: Provides a gentle, sterile environment for the clarification and washing of extracellular vesicles and synthetic nanoparticles.

• Scalable Process Development: Designed to provide linear scalability to larger KrosFlo TFF systems, ensuring that benchtop results translate accurately to full-scale production.

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