Corning Culture Flask 162 cm2 175 ml Vented Cap PS Sterile Case of 25 Flasks

Description

Corning 3151 162 cm² (T-175) Cell Culture Flask (Straight Neck, Vent Cap)

The Corning 3151 is a premium, high-volume cell culture flask designed for the efficient scale-up of anchorage-dependent mammalian cells. With an expansive 162 cm² growth area, this flask is manufactured from high-clarity, medical-grade virgin polystyrene that has been vacuum-gas plasma treated to create a highly hydrophilic Tissue Culture (TC) surface. The straight neck design provides easy access for pipettes and cell scrapers across the entire growth surface, while the specialized vent cap integrates a 0.2 µm pore hydrophobic membrane. This membrane ensures consistent, sterile gas exchange while minimizing the risk of contamination and liquid leakage. Each flask is certified non-pyrogenic and sterile, providing a reliable environment for critical applications in immunology, oncology, and vaccine development.

Technical Performance and Design

• Vacuum-Gas Plasma TC Treatment: The growth surface is permanently modified to ensure consistent, reliable cell attachment and spreading across the entire 162 cm² area, reducing well-to-well and flask-to-flask variability.

• Advanced Vent Cap Technology: The 0.2 µm hydrophobic membrane allows for continuous CO₂ and O₂ exchange even when the cap is fully tightened, providing superior contamination control compared to manual “plug-seal” venting.

• Ergonomic Straight Neck: The wide, straight neck profile is optimized for easy access with large-volume serological pipettes (up to 50 ml) and cell scrapers, facilitating efficient harvest and media changes.

• Enhanced Optical Clarity: The ultra-flat, distortion-free base allows for high-quality microscopic visualization of cell morphology and confluence directly through the polystyrene.

Product Specifications

• Catalog Number: 3151

• Growth Area: 162 cm² (T-175)

• Nominal Volume: 175 ml (Recommended Working Volume: 32 ml to 48 ml)

• Material: High-Clarity Virgin Polystyrene (PS)

• Neck Style: Straight Neck

• Cap Style: Vent Cap (0.2 µm Hydrophobic Membrane)

• Sterility: Sterile (Gamma Irradiated); Non-pyrogenic (< 0.1 EU/ml)

• Packaging: 5 Flasks per Pack / 25 Flasks per Case

Key Benefits & Applications

• Adherent Cell Expansion: The preferred format for scaling up cultures of HEK293, CHO, and Vero cells for protein production or viral vector manufacturing.

• Continuous Incubation: Ideal for long-term experiments where maintaining consistent pH and gas tension is critical, without the need for manual cap adjustments.

• Minimized Risk of Contamination: The hydrophobic vent filter prevents the ingress of microbes and aerosols while also preventing the internal media from wetting the filter and blocking gas flow.

• Stackable Design: Features a rimmed base and stable profile, allowing for secure stacking in the incubator to maximize space efficiency during large-scale runs.

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