Tris-Glycine-SDS Buffer 10X Concentrate, 1 Liter

Description

Description

Tris-Glycine-SDS Buffer 10X Concentrate, 1 Liter

Synonym: TRIS glycine SDS buffer solution

• eCl@ss 32129211
• NACRES NA.25

General description

Tris-glycine-SDS (TGS) running buffer is the most commonly used buffer for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. TGS is usually used for both anode and cathode buffer. Recommended running conditions is 150 volts for mini vertical gel electrophoresis units.

Application

Tris-glycine-SDS buffer 10× concentrate has been used as a running buffer in sodium dodecyl sulfate-polyacryamide gel electrophoresis (SDS-PAGE). It is also used to block membranes with 5% non-fat dry milk for western blotting.

Reconstitution

Dilution of the 10× TGS buffer produces a 1× running buffer containing 25 mM Tris, 192 mM glycine and 0.1% SDS, pH approx. 8.6.

Safety Information

GHS07

Signal word Warning
Hazard statements H319
Precautionary statements P280-P305 + P351 + P338-P337 + P313
Personal Protective Equipment Eyeshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter
RIDADR NONH for all modes of transport
WGK Germany 1

Documents 

Safety Data Sheet