Corning Microplate 96 Well with Lid Clear Bottom PS Case of 100 Plates

Description

Corning 3904 96-Well Black/Clear Bottom Microplate (TC-Treated, with Lid)

The Corning 3904 is a premium 96-well microplate engineered specifically for fluorescence and luminescence assays requiring high-resolution bottom-reading or microscopic imaging. The plate features opaque black walls to virtually eliminate well-to-well crosstalk and background auto-fluorescence, while the ultra-thin, clear film bottom provides exceptional optical clarity. Manufactured from medical-grade virgin polystyrene, the growth surface is vacuum-gas plasma treated for consistent Tissue Culture (TC) performance, ensuring robust attachment and growth of anchorage-dependent cells. Each plate is supplied sterile and non-pyrogenic with an included low-evaporation lid, providing an ideal platform for cell-based screening and high-content analysis.

Technical Performance and Design

• Crosstalk Suppression: The solid black pigment in the plate walls absorbs lateral light transmission, preventing signal bleed-through between adjacent wells, which is critical for the accuracy of high-sensitivity fluorescence assays.

• Optically Clear Bottom: The flat, clear bottom is designed for compatibility with inverted microscopes and bottom-reading multi-mode plate readers, providing a distortion-free view of the cell monolayer.

• Validated TC-Treatment: The hydrophilic surface modification ensures uniform cell distribution and consistent attachment across the entire 96-well format, reducing experimental CV values.

• Low-Evaporation Lid: The included lid features a specialized design to maintain a stable environment, minimizing the “edge effect” by reducing evaporative loss during prolonged incubation periods.

Product Specifications

• Catalog Number: 3904

• Format: 96-Well Plate with Lid

• Color: Black Walls with Clear Bottom

• Surface Treatment: Tissue Culture (TC) Treated

• Well Volume: 360 µl (Total Capacity); 75–200 µl (Working Volume)

• Material: Polystyrene (PS)

• Sterility: Sterile (Gamma Irradiated); Non-pyrogenic

• Packaging: 20 Plates per Pack / 100 Plates per Case

Key Benefits & Applications

• Fluorescence-Based Assays: The industry standard for GFP-reporter assays, FRET, and calcium imaging where low background noise is essential for signal detection.

• High-Content Screening (HCS): Ideal for automated imaging systems and high-throughput screening where clear bottom access and opaque walls are required for high-resolution data acquisition.

• Cell-Based Luminescence: Provides the necessary light-blocking properties for luciferase assays and other glow-luminescence applications that require long integration times.

• Microscopic Visualization: Enables researchers to visually confirm cell confluence and health using standard phase-contrast or fluorescence microscopy without transferring samples.

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