Bovine Serum Albumin Heat Shock Fraction PH 5.2 96%, 10 grams

Description

Description

Bovine Serum Albumin Heat Shock Fraction PH 5.2 ≥96% 10 Gram

Synonym: Albumin bovine serum, BSA, Bovine albumin

• CAS Number 9048-46-8
• EC Number 232-936-2
• MDL number MFCD00130384
• NACRES NA.27

General description

Bovine serum albumin (BSA) is a 66 kDa protein consisting of three domains with two subdomains under each. It is a α-helical, globular and non-glycosylated protein. It belongs to the serum albumins family. It has 17-disulfide bonds.

Application

Bovine Serum Albumin has been used:
• with primary antibodies to prevent non-specific binding in immunohistochemistry of heart sections
• as a component of trypsin inhibitor solution
• as a component of sterile sorting buffer for immunocytochemistry of common lymphoid progenitor (CLP) cells

Packaging

1 kg in poly drum

10, 50, 100, 500 g in poly bottle

Biochem/physiol Actions

Bovine Serum Albumin (BSA) is a transporter for drugs, hormones and fatty acids. It is used as a blocking agent in enzyme linked immunosorbent assay (ELISA) for preventing non-specific binding of antigens and antibodies to the microtiter plates. BSA is a crucial component of the cell culture media and favors embryonic stem cells (hESC) differentiation. It shares structural features with human serum albumin. Its high solubility, low cost and purity and interaction with surfactant is exploited in cosmetic and pharmaceutical industry.

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

Prepared using heat shock fractionation

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term “Fraction V” has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Eyeshields, Gloves, type N95 (US)

RIDADR NONH for all modes of transport
WGK Germany 3

Documents 

Safety Data Sheet