Description
Description
Agarose For Pulsed Field Electrophores, 25 Gram
- CAS Number 9012-36-6
- EC Number 232-731-8
- MDL number MFCD00081294
- PubChem Substance ID 24890707
| biological source | algae (red) |
| form | powder |
| application(s) | electrophoresis: suitable |
| impurities | ≤10% water |
| EEO | 0.04-0.10 |
| transition temp | gel point 38-43 °C |
| gel strength | ≥2000 g/cm2 |
| anion traces | sulfate (SO42-): ≤0.20% |
| foreign activity | DNase, RNase, NICKase, none detected |
Application
Especially formulated for fast resolution of large DNA (10-2000 ·kb) by pulsed-field gel electrophoresis (PFGE) or conventional electrophoresis. Pulsed-field gel electrophoresis was developed in 1984 to deal with “reptation” of large DNA′s whose size surpass the limit of resolution and all migrate at the same rate. Alternately pulsed, orthogonally oriented electric fields are applied across the gel. Every time the direction of the electric field changes, the large DNA′s can no longer migrate until they become reoriented along the new electric field. The time required for reorientation is dependent on the size of the DNA, therefore the DNA is fractionated by size.
Features and Benefits
• Low EEO allows for increased voltages and more rapid DNA migration
• Greater gel strength allows for stability of lower concentration gels leading to faster DNA migration
• Gels exhibit low background fluorescence using ethidium bromide staining
Safety Information
Personal Protective Equipment Eyeshields, Gloves, type N95 (US)
RIDADR NONH for all modes of transport
WGK Germany 3
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