Description
Description
Bovine Serum Albumin Heat Shock Fraction Essentially Fatty Acid Free 98%, 100 Gram
Synonym: Albumin bovine serum, BSA, Bovine albumin
- CAS Number 9048-46-8
- EC Number 232-936-2
- MDL number MFCD00130384
| Quality Level | 200 |
| biological source | bovine |
| assay | ≥98% (agarose gel electrophoresis) |
| form | lyophilized powder |
| mol wt | ~66 kDa |
| purified by | heat shock fractionation |
| packaging | poly bottle of |
| origin | USA origin |
| impurities | ≤0.005% Fatty acids |
| loss | ≤5.0%, Loss on drying |
| pH | 7 |
| solubility | water: soluble (40 mg/ml) |
| suitability | suitable for ELISA applications (as diluent) |
| storage temp. | 2-8°C |
General description
Bovine serum albumin (BSA) is a 66.4 kDa, water-soluble protein composed of 583 amino acids. It has a single polypeptide chain with three homologous domains formed by six α-helices. Depending on pH, it undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating. BSA has been used as a model protein in various applications, including immunodiagnostic procedures, cell culture media and clinical chemistry. Serum albumins facilitates drug disposition and efficacy by maintaining osmotic blood pressure.
Application
Bovine Serum Albumin has been used:
• to prevent nonspecific adsorption of fibrin matrices to tissue culture plates
• as a constituent in the preparation of albumin droplets
• as a supplement in L-15 medium to increase spermatogonia number without enhancing the growth of somatic cells
• as a standard protein to evaluate metal oxide adsorption isotherms
Safety Information
Personal Protective Equipment Eyeshields, Gloves, type N95 (US)
RIDADR NONH for all modes of transport
WGK Germany 3
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