Description

Dextrin Sepharose High Performance has high binding capacity for MBP-tagged proteins. This robust and stable Sepharose High Performance based medium enables elution of target protein in narrow peaks, which minimizes the need for further concentration steps. Purification is performed under physiological conditions and the mild elution preserves the activity of the target protein. The mild conditions even allow purification of intact protein complexes. Due to the high specificity of the binding, very high purity of the eluted protein is achieved in just one step. Dextrin Sepharose High Performance and MBPTrap HP are easily regenerated using .5 M NaOH.