Description
Description
HEPES Solution Bioperformance Certified 1 M 1 Liter
Synonym: N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
- CAS Number 7365-45-9
- MDL number MFCD00006158
- PubChem Substance ID 329815199
- NACRES NA.25
| Quality Level | 200 |
| grade | BioPerformance Certified |
| sterility | 0.2 μm filtered |
| concentration | 1 M |
| application(s) | cell culture | mammalian: suitable |
| impurities | Bioburden, tested |
| DNase, RNase, Protease, Nickase, free | |
| endotoxin, tested | |
| useful pH range | 6.8 – 8.2 |
| cation traces | Fe: |
| heavy metals (as Pb): | |
| suitability | suitable for molecular biology |
| Featured Industry | Diagnostic Assay Manufacturing |
| SMILES string | OCCN1CCN(CCS(=O)(O)=O)CC1 |
| InChI | 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14) |
| InChI key | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
General description
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Application
RNAse HEPES has been used:
• To supplement Dulbecco’smodified Eagle’smedium for maintenance of cell line and RPMI medium to wash rat islets
• To recover purified ribonucleotide
• As a component of HEPES/KOH buffer and adenylation buffer for small RNA isolation and sequencing
• As a component in buffers used for nuclear extract preparation and also to supplement RPMI-1640 medium for maintenance of islets
Documents
Safety Data Sheet
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