Description
Description
Jumpstart Redtaq DNA Polymerase Hot-Start Taq Enzyme 2500 Unit
- MDL number MFCD00166026
- NACRES NA.55
| form | liquid |
| feature | hotstart |
| concentration | 1 unit/μL |
| color | red |
| red | |
| shipped in | wet ice |
| storage temp. | −20°C |
General description
JumpStart REDTaq DNA Polymerase is Sigma’s high performance Taq DNA Polymerase blended with JumpStart Taq antibody and an inert red dye tracer. Extensive testing with a variety of primers and templates indicates that the performance of JumpStart REDTaq DNA Polymerase is equivalent to, or better than, that of standard Taq polymerase.
• The hot start Taq antibody prevents non-specific product formation.
• Assembled PCR reactions can be placed at room temperature up to 2 hours.
• Red tracer allows visible tracking of enzyme addition and reaction mixing.
• PCR can be directly loaded onto an agarose gel without addition of loading buffers.
• The red tracer is a tracking dye that run at 125bp on a 1% agarose gel.
Since the red tracer has no effect on the amplification process, a sample can be easily re-amplified such as in “nested PCR”. The presence of the dye also has no effect on automated DNA sequencing; ligase mediated ligations, exonucleolytic PCR product digestion, and transformation. Though exceptions may exist, the dye is generally inert in restriction enzyme digestions. If necessary, the dye can be removed from the amplicon by routine purification methodologies.
Application
JumpStart™ REDTaq DNA Polymerase has been used in:
• polymerase chain reaction (PCR) for the amplification of insulin-enterotoxin ricin fusion gene (INS-RTB)
• endothelial cells DNA derived from reverse transcribed RNA
• leg genomic DNA from cricket flies
Features and Benefits
• Prevents amplification of nonspecific products, resulting in increased efficiency and higher yields of the desired sequence
• No lengthy activation step is required for enzyme activity to be restored
• Visual confirmation that the enzyme has been added and that proper component mixing of the reaction has occurred
• Samples can be loaded directly onto an agarose gel for electrophoresis with no loading dye additions
GHS07
Signal word Warning
Hazard statements H315-H319-H335
Precautionary statements P261-P305 + P351 + P338
Personal Protective Equipment Eyeshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter
RIDADR NONH for all modes of transport
WGK Germany 3
Documents
Safety Data Sheet
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