Millipore Filter Microplate MultiScreen 96 Well Ultracel-PPB – Pack of 2 Plates

Description

Millipore MultiScreen Ultrafree-PPB Filter Plate – 96-Well, Plasma Protein Binding Assay, Ultracel Membrane

The Millipore MAPPB1002 is a highly specialized 96-well MultiScreen filter plate assembly engineered specifically for high-throughput Plasma Protein Binding (PPB) studies and drug candidate distribution profiling. Unlike traditional, time-consuming equilibrium dialysis setups, this automation-compatible platform features integrated Ultracel® regenerated cellulose membranes calibrated for rapid, centrifugal-based separation of free (unbound) drugs from protein-bound drug fractions in serum or plasma samples. Designed to handle a 500 µL maximum filter well headspace, the single-piece plate configuration exhibits exceptionally low non-specific drug adsorption, delivering the high-precision volumetric recoveries and consistent lot-to-lot reliability demanded by ADME/Tox screening workflows in preclinical drug discovery.

Technical Performance and Design

• Ultracel® PPB Regenerated Cellulose Membrane: Formulated specifically to ensure ultra-low non-specific drug binding. This material ensures that test compounds pass cleanly through the filter barrier without sticking to the polymer, providing highly accurate concentration profiles of free versus bound therapeutics.

• Centrifugal High-Throughput Alternative: Engineered to process up to 96 samples simultaneously via clinical or laboratory swinging-bucket centrifuges. This system drastically compresses the standard 24-to-48-hour timelines required by legacy dialysis methods down to a single spinning cycle.

• Dual-Capacity Volumetric Configuration: Offers a maximum structural filter well capacity of 500 µL. When paired with standard 300 µL receiver microtiter plates, it safely handles a 300 µL working fluid volume, expandable up to 500 µL if matched with an external deepwell collection block.

• Standardized Automation Footprint: Adheres strictly to universal ANSI/SLAS microplate dimensional criteria, guaranteeing seamless alignment with robotic fluid handlers, automated multichannel pipetting heads, and stacker transport grippers.

Product Specifications

• Catalog Number / MPN: MAPPB1002

• Product Family: MultiScreen® Ultracel-PPB

• Format: 96-Well High-Throughput Centrifugal Filter Plate

• Membrane Matrix: Ultracel® Regenerated Cellulose (Optimized for Protein Binding)

• Filter Well Capacity: 500 µL Maximum

• Working Sample Volume: 300 µL (Using standard included receiver plates)

• Centrifugation Limits: 2,000 × g Recommended Operating Force (3,000 × g Maximum Limit)

• Sterility: Non-Sterile

Key Benefits & Applications

• Preclinical ADME / Tox Screening: The industry-baseline standard for determining the plasma protein binding affinity of chemical libraries, a crucial parameter for determining in vivo pharmacokinetic drug distribution and clearance rates.

• Free-Drug Fraction Quantitation: Safely filters out dense serum albumin and alpha-1-acid glycoprotein complexes, leaving behind a clean, protein-free ultrafiltrate optimized for direct injection into downstream LC-MS/MS systems.

• Assay Miniaturization: Minimizes the required volumes of costly test compounds, target proteins, and primary animal serum vectors, maximizing data generation per microgram of analyte.

• Process Automation: Eliminates the tedious manual manipulation, loading, and tracking steps associated with multi-chamber dialyzer membranes, adapting flawlessly to automated liquid-handling workflows.

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