Phenomenex Strata-X-C Microplate 10 mg Strong Cation Each Well 96 Well – 2 Plates

Description

Phenomenex Strata-X-C SPE Microplate – 10 mg/well, Strong Cation, 96-Well

The Phenomenex 8E-S029-AGB is a high-performance Solid Phase Extraction (SPE) microplate engineered for the rapid, high-throughput isolation of weakly basic compounds from low-volume samples. Each well in the 96-well polypropylene manifold contains 10 mg of Strata-X-C, a 33 µm polymeric strong cation-exchange sorbent. Unlike traditional silica-based sorbents, this rugged polymeric backbone resists deconditioning if accidentally dried out during the conditioning or washing steps, entirely eliminating the risk of lost sample recoveries. The uniquely modified mixed-mode chemistry uniformly bonds a sulfonic acid ligand to the polymer, offering three mechanisms of retention (strong cation exchange, pi-pi bonding, and hydrophobic interactions). This strong ionic binding allows researchers to aggressively wash away matrix interferences using 100% organic solvents, resulting in ultra-clean extracts that significantly improve LC-MS/MS sensitivity while utilizing minimal elution volumes due to the highly efficient 10 mg sorbent mass.

Technical Performance and Design

• Mixed-Mode Retention: Synergistically combines strong cation-exchange capabilities with reversed-phase (hydrophobic and pi-pi) bonding, providing unparalleled selectivity and retention for weakly basic analytes (pKa 8-10).

• 100% Organic Wash Capability: The strong ionic binding of target bases enables the use of 100% organic solvents during the wash step, effectively stripping away neutral interferences, phospholipids, and particulates without analyte breakthrough.

• Low Bed Weight (10 mg): The efficient 10 mg sorbent mass is optimized for smaller sample sizes, allowing researchers to utilize highly concentrated, low-volume elution steps that often completely bypass the need for solvent evaporation and reconstitution before LC-MS/MS injection.

• High-Throughput 96-Well Format: Built into a standard SBS-footprint polypropylene plate, making it seamlessly compatible with automated liquid handling robots, positive pressure manifolds, and 96-well vacuum blocks for rapid batch processing.

Product Specifications

• Catalog Number: 8E-S029-AGB

• Format: 96-Well Plate

• Phase/Chemistry: Strata-X-C (Polymeric Strong Cation Mixed Mode)

• Particle Size: 33 µm (85 Å Pore Size)

• Sorbent Mass: 10 mg per well

• Housing Material: Polypropylene

• Target Analytes: Weak Bases (pKa 8-10)

Key Benefits & Applications

• Clinical & Toxicological Assays: The industry standard for the high-throughput extraction of basic pharmaceutical compounds, drugs of abuse, and metabolites from complex biological fluids like blood plasma, serum, and urine.

• Low-Volume Efficiency: Perfect for pediatric testing, murine (mouse) models, or any diagnostic assay where the available biological sample volume is strictly limited.

• LC-MS/MS Optimization: Highly effective at stripping away phospholipids and salts, preventing the severe ion-suppression effects that frequently plague sensitive mass spectrometry detectors.

• Robust Method Reliability: The advanced polymeric backbone prevents the sorbent from collapsing, ensuring that accidental drying under a vacuum manifold will not compromise sensitive extraction recoveries.

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