Description
Description
PIPES≥99% Titration 500 Gram
Synonym: 1,4-Piperazinediethanesulfonic acid, Piperazine-1,4-bis(2-ethanesulfonic acid), Piperazine-N,N′-bis(2-ethanesulfonic acid)
- CAS Number 5625-37-6
- Empirical Formula (Hill Notation) C8H18N2O6S2
- Molecular Weight 302.37
- Beilstein/REAXYS Number 817713
- EC Number 227-057-6
- MDL number MFCD00006159
- PubChem Substance ID 24898793
- NACRES NA.25
| Quality Level | 300 |
| assay | ≥99% (titration) |
| useful pH range | 6.1 – 7.5 |
| pKa (25 °C) | 6.8 |
| mp | >300 °C (lit.) |
| solubility | 1 M NaOH: 20 + 80 mL g, clear, colorless |
| Featured Industry | Diagnostic Assay Manufacturing |
| SMILES string | OS(=O)(=O)CCN1CCN(CC1)CCS(O)(=O)=O |
| InChI | 1S/C8H18N2O6S2/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16) |
| InChI key | IHPYMWDTONKSCO-UHFFFAOYSA-N |
General description
PIPES is a member of the ethanesulfonic acid buffer series, first introduced by Good et al., developed to meet certain criteria: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range and easily synthesized. Since its pKa at 37 °C is near physiological pH, PIPES has applications in cell culture work.
Application
Glutaraldehyde fixation of plant and animal tissue samples can cause loss of lipid, leading to apparent morphological changes. Lipid loss and artifacts were minimized when PIPES was used to buffer the glutaraldehyde fixative.,
Alkaline phosphatase activity is lost selectively from certain rat hepatocyte organelles when fixed for ultracytochemistry with cacodylate-buffered glutaraldehyde. When PIPES was used as buffer, retention of activity was 60% greater.
Fixation of fungal zoospores for fluorescence microscopy and electron microscopy was optimal with a combination of glutaraldehyde and formaldehyde in PIPES buffer.
Documents
Safety Data Sheet
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