Description
Description
PIPESBioXtra ≥99% Titration 250 Gram
Synonym: 1,4-Piperazinediethanesulfonic acid, Piperazine-1,4-bis(2-ethanesulfonic acid), Piperazine-N,N′-bis(2-ethanesulfonic acid)
- CAS Number 5625-37-6
- Empirical Formula (Hill Notation) C8H18N2O6S2
- Molecular Weight 302.37
- Beilstein/REAXYS Number 817713
- EC Number 227-057-6
- MDL number MFCD00006159
- PubChem Substance ID 24898917
| Quality Level | 300 |
| product line | BioXtra |
| assay | ≥99% (titration) |
| impurities | Insoluble matter, passes filter test |
| ign. residue (900°C) | ≤0.5% (as SO4) |
| loss | ≤0.5% loss on drying, 110°C |
| useful pH range | 6.1 – 7.5 |
| pKa (25 °C) | 6.8 |
| mp | >300 °C (lit.) |
| solubility | 1 M NaOH: 0.5 M at 20 °C, clear, colorless |
| anion traces | chloride (Cl-): ≤0.2% |
| cation traces | Al: ≤0.005% |
| Ba: ≤0.0005% | |
| Bi: ≤0.0005% | |
| Ca: ≤0.005% | |
| Cd: ≤0.0005% | |
| Co: ≤0.0005% | |
| Cr: ≤0.0005% | |
| Cu: ≤0.0005% | |
| Fe: ≤0.0005% | |
| K: ≤0.005% | |
| Li: ≤0.0005% | |
| Mg: ≤0.0005% | |
| Mn: ≤0.0005% | |
| Mo: ≤0.0005% | |
| Na: ≤0.1% | |
| Ni: ≤0.0005% | |
| Pb: ≤0.0005% | |
| Sr: ≤0.0005% | |
| Zn: ≤0.0005% | |
| absorption | ≤0.1 at 260 in 1 M NaOH at 0.5 M |
| ≤0.1 at 280 in 1 M NaOH at 0.5 M | |
| Featured Industry | Diagnostic Assay Manufacturing |
| SMILES string | OS(=O)(=O)CCN1CCN(CC1)CCS(O)(=O)=O |
| InChI | 1S/C8H18N2O6S2/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16) |
| InChI key | IHPYMWDTONKSCO-UHFFFAOYSA-N |
General description
PIPES is a member of the ethanesulfonic acid buffer series, first introduced by Good et al., developed to meet certain criteria: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range and easily synthesized. Since its pKa at 37 °C is near physiological pH, PIPES has applications in cell culture work.
Application
Protocols have been reported on the use of PIPES for separation of glyoxylated RNA in agarose gels, nuclease S1 mapping of RNA, and in ribonuclease protection assay protocols. PIPES has been used as a buffer in glutaraldehyde fixation of tissue samples.,
PIPES has been utilized in protein crystallization., The use of PIPES in the reconstitution of dissociated tubulin
α and β subunits after their resolution on immunoadsorbent gels has been described. PIPES has been recommended for use in buffers for the in vitro study of caspases 3, 6, 7, and 8.
A published study demonstrated the usefulness of PIPES as a non-metal ion complexing buffer in such applications as protein assays. PIPES has been used in cell culture for such applications as the engineering of a thermostable mutant membrane protein in Escherichia coli.
Documents
Safety Data Sheet
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