Thermo Scientific BioLite Microplate 96 Well 200 ul PS Case of 50 Plates

Description

Thermo Scientific BioLite Cell Culture Microplate – 96-Well, 200 µL (0.2 mL), Clear Polystyrene, Flat Bottom, with Lid, Sterile (MPN: 130188)

The Thermo Scientific BioLite 130188 is an economy-grade, high-performance 96-well cell culture microplate designed to provide exceptional cost efficiency for routine, everyday anchorage-dependent cell culture and cell-based assays. Molded from premium, crystal-grade USP Class VI virgin polystyrene, this standard-format plate features an 8 × 12 well array with a nominal volumetric capacity of 200 µL (0.2 mL) per well. Each well undergoes a specialized BioLite gas-plasma surface treatment, rendering the hydrophobic plastic permanently hydrophilic to facilitate uniform cell attachment, spreading, and monolayer formation. Shipped fully sterilized and packaged with a matching clear, condensation-ringed lid, this plate delivers reliable performance while keeping laboratory consumables budgets well-optimized.

Technical Performance and Design

• BioLite™ Cell Culture Surface Treatment: Evaluated and validated for routine adherent cell lines, this specialized vacuum-gas plasma treatment introduces oxygen-containing functional groups onto the polystyrene base. This yields a clean, hydrophilic surface that promotes steady cell anchoring and growth.

• Optically Pristine Flat F-Bottom Geometry: Designed with a completely flat well base (F-bottom style), providing a uniform 0.32 cm² culture area. This ensures a consistent focal plane across the entire well surface, optimizing it for high-clarity automated phase-contrast imaging and bottom-read microplate reader optics.

• Evaporation-Reducing Clear Lid: Supplied complete with a matching transparent polystyrene lid featuring built-in condensation rings. This layout aligns directly over individual wells to redirect moisture drop-back, minimizing cross-well contamination and sample evaporation edge effects during prolonged incubation loops.

• Certified Cleanroom Purity: Manufactured in strict compliance with ISO 11137 standards and gamma-irradiated to achieve a 10⁻⁶ Sterility Assurance Level (SAL). Every production lot is certified non-pyrogenic, non-cytotoxic, and free of detectable RNase, DNase, and BSE/TSE trace materials.

Product Specifications

• Catalog Number / MPN: 130188

• Manufacturer / Product Line: Thermo Scientific / BioLite™ (Manufactured by Nunc)

• Format: Standard 96-Well Matrix (8 × 12 Array)

• Well Maximum Capacity: 200 µL (0.2 mL) Total Well Volume

• Growth Area Per Well: 0.32 cm²

• Well Bottom Profile: Flat Bottom (F-Well)

• Material Composition: Crystal-Clear Polystyrene (PS), USP Class VI Raw Material

• Sterility Status: Gamma Irradiated Sterile (SAL 10⁻⁶)

• Compliance Standard: Fully conforms to ANSI/SLAS 1-2004 dimensional microplate footprints

Key Benefits & Applications

• Routine Adherent Cell Maintenance: An exceptionally economical selection for screening common, robust cell lines (such as HeLa, HEK293, and CHO) that do not require premium or proprietary advanced surface coatings.

• Colorimetric & Fluorometric Assays: The high optical clarity of the virgin polystyrene matrix supports precise data acquisition during standard multi-well ELISA protocols, cell viability screenings (MTT/CCK-8), and reporter gene assays.

• Academic & High-Volume Screening Cores: Specifically budgeted to maximize screening volume efficiency in high-throughput university and industrial laboratory lines without sacrificing structural plate uniformity.

• Flawless Automation Deck Fit: The standardized exterior footprint guarantees smooth physical handoffs when interacting with automated robotic plate grippers, liquid handlers, stackers, and standard plate readers.

All product names, logos, and brands are property of their respective owners.