Thermo Scientific Top Works for Nalgene Carboy – 3 Aseptic Closure Systems

Description

Thermo Scientific Nalgene Top Works™ Flexible System (53B Closure, Catalog 2135-5303)

The Thermo Scientific Nalgene Top Works™ 2135-5303 is a premium, “plug-and-play” closure assembly designed to create an instant aseptic fluid transfer system. Unlike standard ported caps that require manual tubing insertion, this system features platinum-cured silicone tubing fused directly into a thermoplastic elastomer (TPE) stopper insert. This “one-piece” integrated construction eliminates the risk of tubing slippage or bypass leakage at the insertion point, providing a significantly higher level of sterility assurance for critical upstream applications. Engineered to fit vessels with a 53B (53 mm) neck finish, this assembly is the standard choice for smaller Nalgene carboys and heavy-duty vacuum bottles.

Technical Performance and Design

• Fused One-Piece Construction: The silicone tubing is permanently bonded to the TPE stopper, creating a seamless, leakproof interface that maintains its integrity even under the thermal stress of repeated autoclaving.

• Versatile 3-Port Configuration: Features a specialized tri-port layout designed for simultaneous fluid management: two high-flow 1/4-inch (6.4 mm) ID lines for inlet/outlet streams and one 1/8-inch (3.2 mm) ID line for venting or sampling.

• Platinum-Cured Silicone Tubing: Utilizes high-purity, ultra-flexible silicone that ensures low extractables and minimal binding of proteins or active pharmaceutical ingredients (APIs).

• Leakproof 53B Interface: Designed with a precision-molded polypropylene screw cap that provides a secure, airtight seal on standard 53 mm neck finishes, making it ideal for vacuum or pressure-driven transfers.

Product Specifications

• Catalog Number: 2135-5303

• Closure Size: 53B (53 mm)

• Port 1 & 2: 1/4-inch (6.4 mm) ID Tubing

• Port 3: 1/8-inch (3.2 mm) ID Tubing

• Materials: Polypropylene (Cap), TPE (Stopper), Platinum-Cured Silicone (Tubing)

• Sterility/Sanitation: Non-sterile; Fully Autoclavable (121°C, 15 psi for 20 min)

• Regulatory: USP Class VI compliant; Non-cytotoxic; Food-grade (CFR 21.177)

Key Benefits & Applications

• Aseptic Inoculation: Facilitates the sterile transfer of seed cultures into larger bioreactors while maintaining a closed system to prevent environmental contamination.

• Media & Buffer Transfer: The two 1/4″ lines allow for efficient, high-volume fluid movement, while the 1/8″ line is ideal for attaching a 0.2 µm vent filter to maintain pressure equilibrium.

• Closed-System Sampling: Allows for the removal of small-volume samples through the dedicated 1/8″ line without disrupting the main process flow or exposing the vessel contents.

• Plug-and-Play Integration: Eliminates the labor-intensive process of fabricating custom carboy caps, providing a validated, off-the-shelf solution that reduces the risk of operator error.

All product names, logos, and brands are property of their respective owners. Nalgene and Top Works are trademarks of Thermo Fisher Scientific.